Department of Biochemistry

Dr. Sibtain Ahmed


Dr. Sibtain Ahmed

Associate Professor, Department of Biochemistry
https://profiles.bzu.edu.pk/sibtainahmed

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Dr. Sibtain Ahmed

Qualification:
PhD Biochemistry

Research Interests:
Natural Products Drug Discovery, Enzyme Biotechnology, Biofuels

Email Address:

Other Information:

Research Papers

2026 Waste-to-resource: Enhanced production of cellulases and lichenase from lignocellulosic biomass via mutated Bacillus subtilis (Journal)
Raza, JA, Aslam, A, Ahmed, S, Andaleeb, H, Waseem, SMY
Journal: International Biodeterioration Biodegradation, Publisher: Elsevier BV, Vol.: 207, Issue: Pages: doi.org10.1016j.ibiod.2025.106227,
2025 Extending Shelf Life of Grapes and Tomato: The Efficacy of Chitosan/PVA Films Strengthened by Terminalia Arjuna Extract and Aloe Vera Gel (Journal Paper)
Shahzad S, Ahmed S, Aslam, A. et al.
Journal: Waste and Biomass Valorization, Publisher: Springer Science and Business Media LLC, Vol.: , Issue: Pages: https.doi.org10.1007s12649-025-03029-1,
2025 Sustainable Biodiesel Manufacturing Utilizing Waste Animal Fats Taken from Slaughterhouses. (Journal Paper)
Arshad M, Ahmed, S, Sultan MD. et al.
Journal: Chemistry Africa, Publisher: Springer Nature, Vol.: 8, Issue: Pages: 2805-2816, https.doi.org10.1007s42250-025-01295-z,
2025 Bioconversion of fruit peels, a waste product of industrial processing into itaconic acid by solid-state fermentation with Aspergillus niger and RSM statistical design to use for optimization process parameters (Journal Paper)
Saeed S, Fatima S, Naseer R, Ahmed S. et al.
Journal: Biomass Conversion and Biorefinery, Publisher: Springer Science and Business Media LLC, Vol.: 15, Issue: Pages: 20095-20106, https.doi.org10.1007s13399-025-06532-,
2025 Rhoifolin Improves Glycometabolic Control in Streptozotocin-Induced Diabetic Rats by Up-Regulating the Expression of Insulin Signaling Proteins and Down-Regulating the MAPK/JNK Pathway (Journal Paper)
Ehsan M, Ahmed S, Majeed W. et al.
Journal: Pharmaceuticals, Publisher: MDPI AG, Vol.: 18, Issue: 3 Pages: 361,
2025 Ascorbic acid-immobilized zinc selenide for electrochemical monitoring of hydrogen peroxide in liver cancer samples (Journal Paper)
Jawad SEZ, Ahmed S, Hussain D, Najeeb J, Alam A, Najum-ul-Haq, Fatima B,
Journal: Scientific Reports, Publisher: Springer Science and Business Media LLC, Vol.: 15, Issue: 1 Pages: 237 doi.org10.1038s41598-024-81411-0,
2025 Exploring cotton plant compounds for novel treatments against brain-eating Naegleria fowleri: An In-silico approach (Journal Paper)
Zaman A, Noor S, Ahmad I, Shehroz M, Alhajri N, Ahmed S, et al.,
Journal: PLOS ONE, Publisher: Public Library of Science (PLoS), Vol.: 20, Issue: 2 Pages: 10.1371journal.pone.0319032 ,
2025 Amalgamation of Piper longum and honey into chitosan as wound dressing material (Journal)
Shahzad S, Ahmed S, Yaqoob A. et al.,
Journal: Chemical Papers, Publisher: Springer Science and Business Media LLC, Vol.: , Issue: Pages: https doi.org10.1007s11696-025-04405-w,
2025 Improved Cellulolytic Activity of Alternaria citri: Optimization and EMS Treatment for Enhanced Cellulase Production (Journal)
Ahmed, S, Andaleeb, H, Aslam, A, Raza, JA, Waseem, SMY, Javaid, A. Talib, C,
Journal: Fermentation, Publisher: MDPI AG, Vol.: 11, Issue: 5 Pages: 274, doi.org10.3390fermentation11050274,
2025 Sericin as Novel Microbial Culture Medium: Proportion Optimization using Response Surface Methodology (RSM) Towards Environmental Health Improvement (Journal Paper)
Mirza SS, Ahmed S., Khalid Y. et al.
Journal: Chemistry Africa, Publisher: Springer Science and Business Media LLC, Vol.: , Issue: Pages: httpsdoi.org10.1007s42250-025-01401-1,
2024 Biopriming of Momordica charantia Seeds with Enterobacter to Improve Nutritional and Biochemical Attributes (Journal Paper)
Shazia Anwer Bukhari, Nabila Farah, Ghulam Mustafa, Sibtain Ahmed, Mohammed Fahad Albeshr
Journal: Journal of Food Quality, Publisher: Wiley, Vol.: , Issue: Pages: ,
2024 Structure based screening and molecular docking with dynamic simulation of natural secondary metabolites to target RNA-dependent RNA polymerase of five different retroviruses (Journal Paper)
Muhammad Azeem, Ghulam Mustafa, Sibtain Ahmed, Amna Mushtaq, Muhammad Arshad, Muhammad Usama, Muhammad Farooq
Journal: PLOS ONE, Publisher: Public Library of Science (PLoS), Vol.: 19, Issue: 8 Pages: ,
2024 Network Pharmacology and Experimental Validation to Explore the Potential Mechanism of Nigella sativa for the Treatment of Breast Cancer (Journal Paper)
Rawaba Arif, Shazia Anwer Bukhari, Ghulam Mustafa, Sibtain Ahmed, Mohammed Fahad Albeshr
Journal: Pharmaceuticals, Publisher: MDPI AG, Vol.: 17, Issue: 5 Pages: 617,
2024 Bioconversion of Mango peels into itaconic acid through submerged fermentation and statistical optimization of parameters through response surface methodology. (Journal Paper)
Saeed S, Ahmed S, Qureshi F, Yasin MS, Waseem R, Mehmood T
Journal: PeerJ , Publisher: Taylor and Francis, Vol.: 12e18188, Issue: Pages: https.doi.org10.7717peerj.18188 ,
2024 Cloning, Expression, and Characterization of a Metalloprotease from Thermophilic Bacterium Streptomyces thermovulgaris (Journal Paper)
Mushtaq A, Ahmed S, Mehmood T, Cruz-Reyes J, Jamil A, Nawaz S
Journal: Biology, Publisher: MDPI, Vol.: 13, Issue: 8 Pages: 619,
2023 Phenolics Extracted from Jasminum sambac Mitigates Diabetic Cardiomyopathy by Modulating Oxidative Stress, Apoptotic Mediators and the Nfr-2/HO-1 Pathway in Alloxan-Induced Diabetic Rats (Journal Paper)
Urooj Umar, Sibtain Ahmed, Asra Iftikhar, Maryam Iftikhar, Wafa Majeed, Atika Liaqat, Sana Shahzad, Mateen Abbas, Tahir Mehmood, Farooq Anwar
Journal: Molecules, Publisher: MDPI AG, Vol.: 28, Issue: 14 Pages: 5453,
2023 Exploring the Potential of Plant Bioactive Compounds against Male Infertility: An In Silico and In Vivo Study (Journal Paper)
Muhammad Jahangeer, Ghulam Mustafa, Naveed Munir, Sibtain Ahmed, Khalid Mashai Al-Anazi
Journal: Molecules, Publisher: MDPI AG, Vol.: 28, Issue: 23 Pages: 7693,
2023 Underlying Mechanisms of Bergenia spp. to Treat Hepatocellular Carcinoma Using an Integrated Network Pharmacology and Molecular Docking Approach (Journal Paper)
Shoukat Hussain, Ghulam Mustafa, Sibtain Ahmed, Mohammed Fahad Albeshr
Journal: Pharmaceuticals, Publisher: MDPI AG, Vol.: 16, Issue: 9 Pages: 1239,
2023 In Silico Analysis of Honey Bee Peptides as Potential Inhibitors of Capripoxvirus DNA-Directed RNA Polymerase (Journal Paper)
Ghulam Mustafa, Hafiza Salaha Mahrosh, Mahwish Salman, Muhammad Ali, Rawaba Arif, Sibtain Ahmed, Hossam Ebaid
Journal: Animals, Publisher: MDPI AG, Vol.: 13, Issue: 14 Pages: 2281,
2023 Valorization of Using Agro-Wastes for Levan through Submerged Fermentation and Statistical Optimization of the Process Variables Applying Response Surface Methodology (RSM) Design. (Journal Paper)
Saeed S, Ahmed S, Naz A, Arooj F, Mehmood T.
Journal: Microorganisms, Publisher: MDPI, Vol.: 6 , Issue: 11 1559. Pages: 1559 ,
2023 Changes in Polyphenols and Antioxidant Properties of Pomegranate Peels Fermented by Urolithin A-Producing Streptococcus thermophilus FUA329. (Journal Paper)
Liu Q, Hua Z, Chen M, Liu S, Ahmed S, et al.
Journal: ACS Food Science and Technology , Publisher: American Chemical Society, Vol.: 8 , Issue: 3 Pages: 1383–1392 ,
2022 Characterization and anticancer potential of Withania somnifera fruit bioactives a native species to Pakistan using gas chromatography-mass spectrometer, nuclear magnetic resonance and liquid chromatography-mass spectrometry-electrospray ionization (Journal Paper)
Tabassam Q, Mehmood T, Ahmed S, Anwar F, Rauf Raza A.
Journal: Journal of Traditional Chinese Medicine, Publisher: China Association of Chinese Medicine and the China Academy of Chinese Medical Sciences, Vol.: 42, Issue: 6 Pages: 908-918,
2022 Discovery of Chitin Deacetylase Inhibitors Through Structure-Based Virtual Screening and Biological Assays. (Journal Paper)
Liu Y, Ahmed S, Fang Y, Chen M, An J, Yang G, Hou X, Lu J, Ye Q, Zhu R, Liu Q, Liu S.
Journal: Journal of Microbiology and Biotechnology, Publisher: Korean Society for Microbiology and Biotechnology , Vol.: 4 , Issue: 32 Pages: 504-513. ,
2022 Valorization of Fruit Peels into Biovanillin and Statistical Optimization of Process Using Enterobacter hormaechei through Solid-State Fermentation. (Journal Paper)
Mehmood T, Ahmed S, Waseem R, Saeed S, Ahmed W, Irfan M, Ullah A.
Journal: Fermentation, Publisher: MDPI, Vol.: 8, Issue: 2 Pages: 40 ,
2021 Expression and characterization of a novel organic solvent tolerant protease from Bacillus sphaericus DS11. (Journal Paper)
Wu X, Ahmed S, Cui X, Hang J, Wang S, Liu S, Fang Y.
Journal: Preparative Biochemistry and Biotechnology, Publisher: Taylor and Francis , Vol.: 1 , Issue: 51 Pages: 28-34. ,
2021 Rationally engineered chitin deacetylase from Arthrobacter sp. AW19M34-1 with improved catalytic activity toward crystalline chitin (Journal Paper)
Zhiwen Ding, Sibtain Ahmed, Jiahao Hang, Haoyu Mi, Xiaoyue Hou, Guang Yang, Zhifa Huang, Xiaoyue Lu, Wei Zhang, Shu Liu, Yaowei Fang
Journal: Carbohydrate Polymers, Publisher: Elsevier BV, Vol.: 274, Issue: 15 Pages: 118637,
2021 Phytochemical investigation and effective therapeutic potential of plants extracts against breast and ovarian cancer cell lines: compounds from zizyphus mauritiana and triticum aestivum (Journal Paper)
Qudsia Tabassam, Sibtain Ahmed, Tahir Mehmood, Mudassir Iqbal, Shagufta Saeed, Muhammad Mushtaq Ahmad, Ayesha Sultan, Muhammad Misbah ur Rehman, Rahman Qadir, Azmat Ullah, Natasha Abdul Rehman
Journal: Cellular and Molecular Biology, Publisher: CMB Association, Vol.: 67, Issue: 1 Pages: 147-152,
2021 GC-MS Metabolomics profiling and HR-APCI-MS characterization of potential anticancer compounds and antimicrobial activities of extracts from Picrorhiza kurroa roots (Journal Paper)
Qudsia Tabassam, Tahir Mehmood, Sibtain Ahmed, Shagufta Saeed, Abdul Rauf Raza, Farooq Anwar
Journal: Journal of Applied Biomedicine, Publisher: University of South Bohemia in Ceske Budejovice, Vol.: 19, Issue: 1 Pages: 26-39,
2021 Structure-Based Designing, Solvent Less Synthesis of 1,2,3,4-Tetrahydropyrimidine-5-carboxylate Derivatives: A Combined In Vitro and In Silico Screening Approach (Journal Paper)
Uzma Arshad, Sibtain Ahmed, Nusrat Shafiq, Zaheer Ahmad, Aqsa Hassan, Naseem Akhtar, Shagufta Parveen, Tahir Mehmood
Journal: Molecules, Publisher: MDPI AG, Vol.: 26, Issue: 15 Pages: 4424,
2021 Isolation, Identification and Sequence Analysis of Subtilisin Gene (Quaking Homolog) Encoding a Fibrinolytic Enzyme from Bacillus subtilis (Journal Paper)
Z. Mushtaq, S. Ahmed, Fateha Irshad, G. Mustafa
Journal: Indian Journal of Pharmaceutical Sciences, Publisher: Indian Pharmaceutical Association - IPA, Vol.: 83, Issue: 4 Pages: 856-864,
2020 Diversity and antimicrobial activity of culturable fungi associated with sea anemone Anthopleura xanthogrammica (Journal Paper)
Liu S, Ahmed S, Zhang C, Liu T, Shao C, Fang Y.
Journal: Electronic Journal of Biotechnology, Publisher: Elsevier, Issue: 44 Pages: 41-46 ,
2020 In silico Study to Reveal Annotation and Significant Interactions of Human Defensin with its Isoforms and their Phylogeny (Journal Paper)
Arif R, Ahmed S, Mustafa, G.
Journal: Indian Journal of Pharmaceutical Sciences, Publisher: Indian Pharmaceutical Association, Vol.: 3 465-471. , Issue: 82 Pages: 465-471 ,
2020 In silico Study to Reveal Annotation and Significant Interactions of Human Defensin with its Isoforms and their Phylogeny (Journal Paper)
R. Arif, S. Ahmed, G. Mustafa
Journal: Indian Journal of Pharmaceutical Sciences, Publisher: Indian Pharmaceutical Association - IPA, Vol.: 82, Issue: 3 Pages: 465-471,
2019 Cloning, Expression and Characterization of a Novel α-Amylase from Salinispora arenicola CNP193 (Journal Paper)
Liu S, Ahmed S, Fang Y.
Journal: Protein Journal, Publisher: Springer Heidelberg, Vol.: 6 , Issue: 38 Pages: 716-722 ,
2017 Fungal Biomass protein production from Trichoderma harzianum using rice polishing. (Journal Paper)
Ahmed S, Mustafa G, Arshad M, Rajoka M I.
Journal: Biomed Research International, Publisher: Wiley Online Library, Vol.: , Issue: 6232793. Pages: 6232793. ,
2016 Phytochemical and Antibacterial Activity of some unexplored medicinal plants of Cholistan desert (Journal Paper)
Mustafa, G, Ahmed S, Ahmed N, Jamil, A.
Journal: Pakistan Journal of Botany, Publisher: Pakistan Botanical Society, Vol.: 5 , Issue: 48 Pages: 2057- 2062 ,
2014 Recombinant expression and characterization of a novel endoglucanase from Bacillus subtilis in Escherichia coli. (Journal Paper)
Zafar M, Ahmed S, Khan MIM, Jamil A.
Journal: Molecular Biology Reports, Publisher: Springer Heidelberg, Vol.: 5 , Issue: 41 Pages: 3295-3302.,
2014 Kinetics and thermodynamics of ethanol production by a Saccharomyces cerevisiae MLD10 using molasses. (Journal Paper)
Arshad M, Ahmed S, Zia MA, Rajoka MI.
Journal: Applied Biochemistry and Biotechnology, Publisher: Springer Heidelberg, Vol.: 5 , Issue: 172 Pages: 2455-2464 ,
2013 Kinetic and Thermodynamic Characterization of Lysine Production Process in Brevibacterium lactofermentum (Journal Paper)
Sibtain Ahmed, Munazza Afzal, Muhammad Ibrahim Rajoka
Journal: Applied Biochemistry and Biotechnology, Publisher: Springer Science and Business Media LLC, Vol.: 170, Issue: 1 Pages: 81-90,
2013 Optimization of antioxidant exopolysaccharidess production by Bacillus licheniformis in solid state fermentation. (Journal Paper)
Fang Y, Ahmed S, Liu S, Wang S, Lu M, Jiao Y.
Journal: Carbohydrate Polymers, Publisher: Elsevier, Vol.: 2 , Issue: 98 Pages: 1377-1382 ,
2013 A directed intimin insertion mutant of a rabbit enteropathogenic Escherichia coli (REPEC) is attenuated, immunogenic and elicits serogroup specific protection. (Journal Paper)
Ahmed S, Byrd W, Kumar S, Boedeker EC.
Journal: Veterinary Immunology and Immunopathology, Publisher: Elsevier, Issue: 152 Pages: 146-55. ,
2012 Comparative study for the kinetics of extracellular xylanases from Trichoderma harzianum and Chaetomium thermophilum. (Journal Paper)
Ahmed S, Imdad SS, Jamil A.
Journal: Electronic Journal of Biotechnology, Publisher: Elsevier, Vol.: 15, Issue: 3 Pages: doi: 10.2225/vol15-issue3-fulltext-2 ,
2012 Production of microbial biomass protein from mixed substrates by sequential culture fermentation of Candida utilis and Brevibacterium lactofermentum. (Journal Paper)
Rajoka MI, Ahmed S, Athar M, Hashmi AS.
Journal: Annals of Microbiology., Publisher: Springer Heidelberg, Vol.: 62, Issue: 3 Pages: 1173-1179 ,
2010 Production of Microbial biomass protein by sequential culture fermentation of Arachniotus sp. and Candida utilis. (Journal Paper)
Ahmed S, Ahmad F, Hashmi AS.
Journal: Pakistan Journal of Botany, Publisher: Pakistan Botanical Society, Vol.: 2 , Issue: 42 Pages: 1225-1234 ,
2009 Production and purification of cellulose-degrading enzymes from a filamentous fungus Trichoderma harzianum. (Journal Paper)
Ahmed S, Bashir A, Saleem H, Saadia M, Jamil A
Journal: Pakistan Journal of Botany, Publisher: Pakistan Botanical Society, Vol.: 3 , Issue: 41 Pages: 1411-1419.,
2009 Caldanaerovirga acetigignens gen. nov., sp. nov., an anaerobic xylanolytic alkalithermophilic bacterium isolated from Trego Hot Spring, Nevada. (Journal Paper)
Wagner ID, Ahmed S, Zhao W, et al.
Journal: International Journal of Systematic and Evolutionary Microbiology, Publisher: Microbiology Society, Vol.: 11 , Issue: 59 2685-2691. Pages: 2685-2691 ,
2008 Regulation of Endo- β-D- Xylanase and β- Xylosidase synthesis in Humicola lanuginosa. (Journal Paper)
Irshad M, Ahmed S, Latif F, Rajoka MI.
Journal: Journal of the Chemical Society of Pakistan, Publisher: Chemical Society of Pakistan , Vol.: 6 , Issue: 30 Pages: 913-918 ,
2007 Xylanase from Trichoderma harzianum: Enzyme characterization and gene isolation. (Journal Paper)
Ahmed S, Jabeen A, Jamil A.
Journal: Journal of the Chemical Society of Pakistan, Publisher: Chemical Society of Pakistan , Vol.: 2 , Issue: 29 Pages: 176-182 ,

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